Tomato (Solanum lycopersicum L.) is the most important vegetable in the world. This species is generally affected by parasitic nematodes which are responsible for crop yield losses ranging between 25% and 50%. Similarly, tomato is affected by pathogens such as the Chromista Phytophthora infestans (Mont.) De Bary, known worldwide by the devastating effects it has produced throughout history and its difficulty to control. The resistance of 15 introductions of wild cherry tomato against late blight (P. infestans) tomato and the root-knot nematode (Meloidogyne spp.) was evaluated in two production systems at the Montelindo farm of Universidad de Caldas using a split-plot experimental design in which the variables evaluated were: Late blight severity and rate of development, index of damage caused by the root-knot nematode and estimated yield (t/ha). The results showed that the introductions evaluated presented susceptibility to both, late blight and root-knot nematode, with average severity of 41,5% for late blight and 8,3% for Meloidogyne spp., equivalent to an average of 30.4 galls per plant. Despite presenting the symptomatology of the diseases evaluated, all the introductions reached production being among the most prominent IAC 421, IAC 1621, IAC 391 and IAC 1688 with yields of 14, 13, 12 and 12 t/ha, respectively.