This work is focused on the synthesis of a biocatalyst consisting of immobilized β-glucosidase onto a superparamagnetic nanomaterial for its application in the production of fermentable sugars. β-glucosidase acquired from Novozymes ® was immobilized on functionalized magnetite nanoparticles by covalent binding. The nanoparticles were synthesized through the co-precipitation technique and were characterized using X-ray diffraction (XRD) and atomic force microscopy (AFM). Magnetite superparamagnetic nanoparticles were obtained lower than 50 nm. The immobilization of β-glucosidase was confirmed using FT-IR spectrophotometry. The activity of the composite was determined, obtaining that approximately 122 units of the enzyme was immobilized per 100 milligrams of nanoparticle used (at pH 4,00 and 60,0 °C). In addition, the immobilized enzyme showed greater activity at pH 5.0 and at a temperature of 60 ° C. The activity retention capacity was measured from studying the composite under five cycles of enzymatic hydrolysis of cellobiose. It was observed that after the fifth cycle of hydrolysis, the immobilized enzyme showed a relative activity of approximately 80% with respect to the first cycle.