Introduction: Suicide is defined by World Health Organization as the deliberate act of taking one's own life. Suicidal behavior covers a wide spectrum, from suicidal ideation to attempt and completion of act. Objective: To determine effects of an educational intervention on level of knowledge of suicidal behavior in adolescents and young people. Methods: We conducted an educational intervention study aimed at adolescents and young people, belonging to health area of Bayamo Oeste teaching polyclinic, in period between April 2010 and March 2011. Through questionnaires, we identified suicidal risk within participants, as well as level of knowledge about subject; from this, educational teaching activities were developed with topics related to suicide. The data obtained were processed through SPSS and absolute frequencies and percentages were used as summary measures of quantitative variables. Results: 11 participants with suicidal risk were identified. More than 80% of study group showed new knowledge after educational intervention was applied. Conclusions: The educational intervention was designed and effectively applied which allowed to increase level of knowledge about suicidal behavior in adolescents and young people.