Bioactive compounds have innumerable properties, with beneficial actions in the treatment of various diseases. In this study, we sought to characterize the genotoxic effect of 6-shogaol in cell cultures in the HT-1080 tumor line; for the above, the viability was evaluated by means of trypan blue and the comet test was used. This test is based on exposing the cells to the compound in a lysis solution: afterwards, the alkaline unwinding was carried out for 30 minutes and the electrophoretic run was carried out for 30 minutes (constant 300mA, 25V). At the end of the run, each sheet was placed in neutralization solution for 15 minutes and dehydrated in methanol. For reading, they were stained with hydragreen 5X and read under the fluorescence microscope (20X). 50 cells were taken per sheet for analysis by the ImageJ Software and the OpenComet pluggin The statistic was made using GraphPad-Prisma 6. As results, it was obtained that at 6h and 12h there is no significant difference between the treatment and the negative control. At 24 and 48 hours. It was observed that the effect is concentration-dependent with significant effects. To conclude, it was found that the exposure time and the concentration with the greatest genotoxic effect was 40M at 24 hours. Likewise, the cells exposed to the different concentrations carried out in this study do not show any effect of 6-Shogaol on the viability of the HT-1080 tumor line