Introduction: invasive mycoses caused by Candida spp remain the most diagnosed in neonatal, pediatric and adult patients hospitalized in intensive care rooms at the provincial level. Objective: to identify the main type of sample by age group and Candida species by type of sample in neonates and children under 18 years. Method: a descriptive, cross-sectional study on the isolates of Candida spp in blood cultures, urine cultures, cerebrospinal fluid, endotracheal tube and catheter tip obtained from neonatal, pediatric and adult patients hospitalized in serious care rooms in the five provincial hospitals of Villa Clara between on January 1, 2018 until August 31, 2019. The sample was 114 positive isolates. Absolute, relative, percent, and statistical frequency distributions were obtained as Pearson's chi-square. Results: adults provided higher numbers of positive isolates, followed by neonates and pediatric age, mainly in 2018. Blood samples, followed by urine samples in neonates and pediatric patients were the most diagnosed; in adults it was the other way around. In neonates Candida tropicalis and Candida spp were the most isolated species, without statistical significance, in pediatric patients were Candida spp and Candida krusei , both with statistical significance in blood cultures and catheter tip. Conclusions: invasive candidiasis was diagnosed in the three age groups, mainly in blood and urine, and with variability in Candida species.