Background: Enteroccocus faecalis is associated with treatment failure due to its ability to develop a biofilm in dentin, presenting resistance to sodium hypochlorite in different concentrations as a substance used in the irrigation process and to calcium hydroxide used as an intracanal medication, this resistance increases as the biofilm matures, contributing to the predominant role of Enteroccocus faecalis in persistent periapical infections. The difficulty in treatment against Enteroccocus faecalis has been attributed to the lack of strategies to prevent biofilm formation and eradicate it. In the search for other treatment strategies, bacteriophages have been tested capable of incapacitating biofilm-forming bacteria, or the prevention of bacterial proliferation through its lysis. Purpose: Detect lytic phages against Enteroccocus faecalis in water samples from the Bogota river. Methods: An experimental descriptive cross-sectional study was carried out, and of a qualitative nature, applying convenience sampling to search for phages with lytic capacity. The double agar layer 28 method was used, and 10 strains of Enteroccocus faecalis of endodontic origin from a previous study were tested (each in triplicate), with 5 water samples from the lower Muna source Basin, and 5 water samples from the El Cortijo half source basin. In addition, trials were conducted to evaluate the lytic capacity against control strains of the species under study, such as Enterococcus faecalis ATCC 29212 y Enterococcus faecalis ATCC 700802, and other species found in the mouth such as Staphylococcus aureus (ATCC 29213), Escherichia coli (ATCC 25922), Streptococcus mutans (ATCC 25175), Leptotrichia bucalis (ATCC 14201). Results: In the middle basin the tests carried out were negative for all strains tested and no lytic phage were found against the Enteroccocus faecalis samples or against the control ATCC strains used. In the lower basin the tests allowed to observe that in two of the samples of Enteroccocus faecalis evaluated lytic phages were found. Staphylococcus aureus (ATCC 29213), y Streptococcus mutans (ATCC 25175). Phage lysed ATCC strains Staphylococcus aureus (ATCC 29213), y Streptococcus mutans (ATCC 25175). Conclusion: The phages found are not specific, they are considered to be broad spectrum, but since they only lysed Gram positive bacteria, it is probably a phage that acts on teicoic acid that is the normal molecule of phage-positive phage adhesion.