The need to regulate livestock activities is indisputable, supported by a regulation that ensures the application of Good Livestock Practices (GLP), based on a clear diagnosis made in the field with producers that show the reality of daily basis. The current research generated information on the levels of compliance of the GLP in selected farms of the northern area of ​​Manabi - Ecuador, applying a stratified random sampling design to implement the diagnosis and to obtain the information through a good practice protocol, which was used to collect the information, which considered necessary elements for GLP compliance; the main aspects were general management of the Agricultural Production Unit (APU), animal health management, veterinary drugs and biological products, feeding and animals catering, environment and infrastructure and animal welfare, during 12 months of monitoring, 60 farms . The level of compliance of the protocol was evaluated according to the total score obtained. For this, the compliance levels used as criteria were: High, Medium, Low. Compliance was distributed in different proportions, the level of high compliance was evidenced in 19 farms (31.67%), the average level in 6 (10%); and, in 35 farms, a low compliance level was evident (58.33%). The components that were most violated belong to the aspects of animal welfare, environment and infrastructure; and, veterinary medicines and biological products; and, the components that were fulfilled correspond to zoosanitary management, feeding and animals watering and general management of the agricultural production unit. The low application of the GLP was demonstrated in a general way, the relation of the balance between the animal - man - environment is not fulfilled.