Rickettsia is a genus consisting of obligate intracellular bacteria; the main vectors are arthropod ectoparasites, espe-cially ticks. In 2008, was detected in Toluviejo, Colombia, a seropositive person to Rickettsia, however, there is little information about the prevalence in this municipality.Objetives. To study the seroprevalence of Rickettsia of the spotted fever group and it’s relation with socio-demographic factors in a human population in Toluviejo, Colombia.