Pestalotiopsis, produced by the fungus Pestalotia palmarum, is the main disease that causes defoliation in oil palm crops in the tropics. This disease considerably affects the sustainability of the crops and their conventional treatment, implies damage to the environment by the pollution it generates. For this reason, the search for innovative natural solutions to this problem in the agro-industrial sector is of great importance, as an alternative to chemical treatment. The adaptability of endophytic fungi to their hosts, the ecological benefits they provide and the various antagonistic mechanisms they have against pest control, make them a good alternative. In this work, the antagonistic capacity of the endophyte fungi isolated from Moringa oleifera was evaluated in the control of Pestalotia palmarum, making use of methods of isolation, identification and evaluation of the antagonism in conjunction with statistical analysis tools, which allowed determining if some of endophytic fungi, could mean an alternative for the biocontrol of Pestaloptiosis. The potential of 21 endophytic fungal strains obtained from healthy tissue was determined as candidates for the biological control of P. palmarum. Of these isolates, eight fungal strains were the most promising. Standing out among these, for its in vitro antagonistic activity against the pathogen, strain MT17, corresponding to Trichoderma sp with 87% inhibition and MH5, corresponding to Curvularia sp with 81% inhibition.