The increased incidence and diversity of fungal infections, and increased resistance to conventional treatments has aroused the interest of the health community. There are few studies developed to know the profiles of in vitro susceptibility of environmental strains against clinical strains, for this reason the objective of the study Is the susceptibility profile of Aspergillus spp., From clinical and environmental isolates of a hospital center Fourth level of complexity. The E-test technique was used to evaluate the minimum Inhibitory concentration (MIC) and the minimum effective concentration (MEC) in vitro for amphotericin B, voriconazole, Itraconazole, caspofungin and anidulafungin with forty five isolates of the genus Aspergillus spp. In the environmental samples the only species of fungi isolated was A. fumigalus, 32% of the isolates of resistance to voriconazole and itraconazole. As for the clinical isolates the most isolated fungus. A. flavus, of which 74% were sensitive to amphotericin B and 26% presented intermediate susceptibility to this antifungal. For A. fumigalus and A. terreus, 100% of the isolates were sensitive. The resistance presented in the environmental isolates is a warning sign because it indicates that they can acquire constant resistance by the constant pressure of the azoles used in agriculture, because they are structurally similar to the medical triazoles and active against Aspergillus fumigalus