In Colombia, the annual production of different types of cereals is around 14'974'807 tons and there are around 71'943'813 tons of post-harvest residues, which contain lignocellulose of difficult degradation. This waste is a problem for farmers because the alternatives that are handled can be expensive or cause damage to the soil or the environment. In the search for solutions to treat waste are the biological alternatives, this is how there are groups of fungi with the ability to degrade these waste thanks to its enzymatic complex. This work was carried out in order to evaluate the viability and the lignocellulolytic activity of 40 filamentous fungal isolations preserved in the bank of the Biotechnology laboratory of the Pontificia Universidad Javeriana. Materials and methods. The reactivation of each isolation of the bank was carried out to verify its viability, for its later conservation. The measurement of the cellulolytic activity was also carried out qualitatively and quantitatively by liquid cultures using rice chaff and carboxymethylcellulose as carbon sources. The FPasas technique (50 ° C and pH 5) was used for the quantification of cellulolytic activity. Of the 40 isolations of filamentous fungi, 55% is viable, where 47.83% belong to cellulolytic fungi and 7.17% to ligninolitic fungi, showing that the conservation method used was effective in the case of fungi with cellulolytic activity, and that for the conservation of fungi with ligninolytic activity, other types of methods must be used and the conservation banks must be constantly monitored. The qualitative evaluation showed that 22 of the 23 isolates evaluated retained their cellulolytic or ligninolytic activity. Finally, in the quantitative evaluation the highest value of enzymatic activity was obtained with the isolation corresponding to Talaromyces sayulitensis with 48.41 ± 15.7 and 38.49 ± 0.7 UC * L-1 for rice chaff and carboxymethylcellulose respectively.