Anthracnose is a fungal disease that has an important incidence in tree tomato crops. It is caused by thephytopathogens Colletotrichum gloeosporioides (Penz.) Or its teleomorph Glomerella cingulata (Stoneman). Thepresent investigation evaluated the antifungal capacity of propolis, a derivative of apicultural products, obtained inthree different locations in Norte Santander (Oripaya, San Cayetano, Los Patios). Initially, a characterization of theresin was carried out by identifying its physicochemical properties, such as: ash 3.56 ± 0.64%; humidity 5.11 ±0.11%; insoluble material 5.11 ± 0.11%; oxidation index 49 ± 1%; melting points 80.90 ± 3.21; UV absorptionspectrum in the range of 270 to 350 nm such as: ash, moisture, insoluble material, oxidation index, melting points,UV absorption spectrum and subsequently, the percentage of inhibition of microorganisms was verified using thetechnique of antibiotic sensitivity test with three concentrations of ethanol extract of propolis EEP (10%, 20%,30%). It was found that the propolis comply with international standards for the parameters of ash, humidity,melting points and absorption spectra of ultra violet (UV) radiation, evidencing the quality of propolis and thepresence of flavonoids, besides it was found that the propolis of the locality of Oripaya at 10% has greater capacityof inhibition against C. gloeosporioides (Penz.)