El estudio de la composicion quimica de los extractos alcoholicos secos de las partes aereas de las especies Ageratina jahnii (B. L. Rob) R. M. King & H. Rob. y Ageratina pichinchensis (Kunth) R. M. King & H. Rob., mediante el uso de la cro-matografia en columna, en capa fina y en capa preparativa, asi como, de las tecnicas espectroscopicas de infrarrojo, ultravioleta, resonancia magnetica nuclear (uni y bidimensional) y espectrometria de masas, permitio el aislamiento e identificacion de seis productos naturales. De A jahnii se aislaron cinco compuestos: dos diterpenos denominados acido ent-kaur-16-en-19-oico y ent-kaur-16-en-3a-ol; tres flavonoides identificados como 5-hidroxi-7,4'-dimetoxiflavona, 5,7-dihidroxi-4'-metoxiflavona y 5,4'-dihidroxi-7-metoxiflavona. Ademas, de A. pichinchensis se aislo el 6-acetil-7-hidroxi-2,2-dimetil-8-metoxi-2H-1-cromeno. Adicionalmente, a partir del ent-kaur-16-en-19-oico; 5-hidroxi-7,4'-dimetoxiflavona y 6-acetil-7-hidroxi-2,2-dimetil-8-metoxi-2H-1-cromeno, se prepararon cuatro derivados quimicos: ent-kaur-16-en-19-oato de metilo; 5-acetoxi-7,4´-dimetoxiflavona; 6-acetil-7-acetoxi-2,2-dimetil-8-metoxi-2H-1-cromeno y 6-acetil-7-hidroxi-2,2-dimetil-8-metoxi-3-yodo-2H-1-cromeno; este ultimo reportado por primera vez segun la base de datos Science Finder. The phytochemical study of dried alcoholic extracts from aerial parts of Ageratina jahnii (B. L. Rob) R. M. King & H. Rob. and Ageratina pichinchensis (Kunth) R. M. King & H. Rob., based on column chromatography, thin layer chromatography and preparative separations, as well as by characterization using infrared, ultraviolet, nuclear magnetic resonance (one and two dimensional) and mass spectrometry leaded the isolation and identification of six natural products. From A. jahnii, five compounds were isolated: two diterpenes known as ent-kaur-16-en-19-oic acid and ent-kaur-16-en-3a-ol; three flavonoids identified as 5-hydroxy-7,4'-dimethoxyflavone, 5,7-dihydroxy-4'-methoxyflavone and 5,4'-dihydroxy-7-methoxyflavone. Furthermore, from A. pichinchensis, 6-acetyl-7-hydroxy-2,2-dimethyl-8-methoxy-2H-1-chromene was cha-racterized. In addition, from the following natural compounds ent-kaur-16-en-19-oic acid, 5-hydroxy-7,4'-dimethoxyflavone and 6-acetyl-7-hydroxy-2,2-dimethyl-8-methoxy-2H-1-chromene; four chemical derivatives were ob-tained: methyl ent-kaur-16-en-19-oate, 5-acetoxy-7,4´-dimethoxyflavone, 7-acetoxy-6-acetyl-2,2-dimethyl-8-methoxy-2H-1-chromene and 6-acetyl-7-hydroxy-3-iodine-2,2-dimethyl-8-methoxy-2H-1-chromene. According to Science Finder data base, the last compound was prepared for first time in this investigation.