The temporary immersion system, known by its acronym (SIT), has emerged as a new alternative for the micropropagation of plant tissues, providing this high rate of multiplication and providing a higher quality to the plant in its acclimatization phase, therefore, the objective of this study, was based on the standardization of a temporary immersion system for the micropropagation of the orchid Cattleya schroederae in the plant tissue laboratory of the University of Santander. For this, the operational conditions of the temporary immersion system were established, where after that, two variables were evaluated, the time and frequency of immersion. The times for SIT-1, was an immersion frequency of 6 hours (4 times / day) with a dive time: 3 minutes, for SIT-2, an immersion frequency of 4 hours (6 times / day) with an 8-minute immersion time and SIT-3, an immersion frequency of 12 hours (2 times / day) and an immersion time of 20 minutes. The study was evaluated over seven weeks, showing that the best response in number of shoots, number of leaves, height and weight, was obtained with the treatment number one, which was working with an immersion frequency every 6 hours (4 times / day) and one immersion time: 3 minutes. Likewise, there was no hyperhydricity or oxidation between the treatments. These results allow establishing an efficient methodology for the micropropagation of Cattleya schroederae through the use of a bioreactor system.