Introduction: The HapMap and the 1000 Genomes projects have been important for understanding the genetic component of common diseases and normal phenotypes. However, the Colombian genetic variability included in these projects is not fully representative of our country. Objective: To contribute to the knowledge of the Colombian genetic variability through the genomic study of a sample of individuals from Bogota. Materials and methods: A total of 2,372,784 genetic markers were genotyped in 32 individuals born in Bogota and with parents from the same region using the Illumina platform. The genetic variability levels were determined and compared with the data available from other populations of the 1000 Genome project. Results: The genetic variability detected in the individuals from Bogota was similar to those with shared ancestry. However, despite the low levels of genetic differentiation between Bogota (CLB) and Medellin (CLM) populations the PCA analysis suggest a different genetic composition between them. Conclusions: Our genomic analysis of a Bogota sample allowed us to detect similarities and differences with other American populations. The increase of the Bogota sample and the inclusion of samples from other regions of the country will allow us to improve our understanding of the genetic variability in Colombia, essential for studies of human health, prevention and treatment of common diseases in our country.