Microalgae and cyanobacteria have been studied extensively in order to produce compounds that are of interest in large industries: food, pharmacology, cosmetics, aquaculture among others; This, thanks to its potential uses in the production of biodiesel, treatment of wastewater, preparation of pigments for food, antioxidants for the human diet, among many others. Within the species of interest, the microalga Spirulina sp. It is recognized for its high nutritional level and the production of compounds such as phycocyanin, a pigment with various antioxidant properties, which has been used for the treatment of diseases and even as a dye in the food industry. In this work, phycocyanin was obtained from the biomass of Spirulina maxima maintained in a PBR Tubular photobioreactor for 15 days, obtaining 4 g / L of biomass; for this, 3 extractions were carried out with 1 M pH 7 phosphate buffer, and 20% ammonium sulfate was used for the purification. Likewise, spectrometric measurements were made to determine the concentration of phycocyanin and allophycocyanin present in the sample, obtaining 0.48 mg / mL of phycocyanin in 150 mg of evaluated biomass, then qualitative analysis, solubility tests, determination of functional groups: amines, aldehydes and ketones, and finally a thin-layer chromatography,finding an ideal retention factor of 0.3 and using as a diluent petroleum ether and ethyl acetate in a 5: 1 ratio for further characterization of column chromatography.