In this study, reducing sugars production by LuffaCylindrica (LC) hydrolysis was evaluated. The celluloseand lignin content (82.54% and 9.73%) of rawmaterial, was determined and reduced in size by acutter mill to a size of 100 mesh. The maximumreducing sugar content obtained was 1.207 mg/mL, 1.493 mg/mL and 2.441 mg/mL for acid, basicand enzymatic hydrolysis respectively. The optimalconditions of pH, time and temperature foracid hydrolysis ( pH 4.0, 60 min, 150°C), alkalinehydrolysis (pH 10, 30 min, 112,5 °C) and enzymatichydrolysis to pH, amount of cellulose and temperature(pH 6.2, 2.39 mL, 42.4°C) were determinedby a design type Box-Behnken. Fermentation studieswere performed with immobilized SaccharomycesCerevisiae at different conditions in order to validatemodels obtained for each type of hydrolysisand achieved yields of 10% ethanol content fortreatments studied.