Dental crown traits (DCT) are phenotypic characteristics of tooth enamel that have expression variability in individuals. Eight DCT and sizes of permanent teeth indigenous of Cauca, Colombia are described. A cross-sectional descriptive study was performed on a sample (n = 100) of indigenous from Guambia (Cauca). Inclusion criteria were applied and signed approval of voluntary consent. Cast models were obtained to analyze eight DCT according to the method of ASUDAS (Central incisors in shovel-shape and double shovel-shape, distal accessory ridge, Carabelli trait, metaconule and cusp 6). Meso-distal and buco-lingual measurements were obtained. The most common trait (47.31%) is shovel-shape in upper incisor. The first molar has contralateral dimorphism (p=0.040) and statistically significant differences in size (p=0.012). The first lower molar was differences in size between contralateral teeth (p=0.029). These results indicate that the DCT are bilateral expression with except Carabelli´s trait. The degree of expression may be related to dental sizes in central incisors and molars.