The traditional food chain is a direct interaction between predators and preys, where a top predator can positively affect basal species due to the control the predators over the other preys. In that section, a part of the energy in one level to another is believed get lost however thank to the detritivore food chain concept and "microbial ring", have been reported that is recycled and reincorporated into the cycle of matter and energy by bacteria in three steps: (1) Processing and decomposition of organic matter, (2) remineralization of nutrients and (3) other food trophic levels. Despite its importance, little is known of its structure in high Andean tropical ecosystems, for this reason, the aim of this study was to evaluate the bacterioplanctónic variability in Andean lakes of Boyaca in three climatic seasons. Triplicate samples were taken in three zones on each lake, surface and coastal background in Laguna Cristalina, Negra and Verde-Boyacá and determined biomass, abundance and bacterial forms and physico-chemical variables (nitrites, nitrates, phosphates, organic matter, temperature, pH, conductivity, oxygen) and chlorophyll α. We found that bacterial abundance and biomass are within the range for oligotrophic. Bacillary forms are most common in these ecosystems and Chlorophyll α is important part to explain the structure of bacterioplankton by the correlation of input of organic matter by phytoplankton and nutrient remineralization by bacteria. It was concluded that phosphorus could be a limiting nutrient from the bacterioplankton.