This master thesis has two components. The first is the effect study of formulation variables on microstructure and macroscopic properties of Kefiran films (Kefiran is an exopolysaccharide obtain from Kefir´s grains gown in milk). The second is application study of Kefiran films formulation like coatings over skin of Colombian creole mangoes. The effect of glycerol into microstructure of Kefiran was evaluated in the order to contribute to fundamental understanding of their effect on barrier and mechanical properties of films based on this. Kefiran films with glycerol addition were characterized thought termogravimetric analysis (TGA) with gas mass spectroscopy (MS), differential scanning calorimetric (DSC), tensile properties and water vapor permeability (WVP) measurement. The findings of this study showed that glycerol has two different effects depending their addition level into Kefiran films; An antiplasticizer effect to low levels (?10%) and plasticizer effect to high levels (15%?). The film with 10% of glycerol addition has the lowest WVP. The film with 25% of glycerol addition has similar tensile properties that low density polyethylene (LDPE). The effect of citric acid and chitosan addition into Kefiran films formulation was evaluated with WVP and tensile properties characterization. Also their antibacterial effect against Escherichia Coli. The addition of citric acid 25% (w/w) into Kefiran films reduces film´s WVP and increases film´s mechanical resistance. The addition of chitosan 33% (w/w) into Kefiran films gives to these antibacterial effect against E.Coli. The effect of Kefiran coating with or without glycerol content into properties of Colombian cleole mangoes was studying. Carbohydrates (fructose and sucrose) and ethanol concentration of mangoes was determinate by liquid chromatographic (HPLC). The carbon dioxide (CO2) production and color change of mangoes were monitoring under hermetic storage, as well as mass loss under open storage.