Bio-digestion is a strictly anaerobic reaction in which organic matter produces biogas and other by-products of added value such as fertilizers. Due to taking advantage of organic waste and its cheap means of production, the usage of biofuels like biogas has been strengthened in the last years. This project aims to design a lab scale bio-digestion system, promoting research in removable energies in the Jorge Tadeo Lozano University, and it’s based on the biodigester design of the Hochschule Offenburg university of applied sciences, utilizing cow manure (CM) and restaurant wastes as inoculum material (IM) as waste, looking to control design variables such as temperature, pH, and manure:water (M:W) dilution. Through Fourier Transform Infrared Spectroscopy tests to the samples before and after the bioreaction, typical functional groups from phases of bio-digestion like hydrolysis and acidogenesis are identified. From characterization to biomass, enrichment in certain nutrients was observed, which influences the viability for it to be used as manure. The design shall be modified in order to correct design errors and to achieve a more sophisticated measurement.