A study on the incidence of health care infection at Children's Hospital Dr. Elias Toro IVSS was performed since July 2013 to July 2014. There were 69 cases of infected patients among 6,107 cases that were tested, so the rate of infection was 1.12 per 100 infection discharges. October and November 2013 were the months in which the frequency of IAAS was increased. Infants and male were the most frequently affected group of age and sex. The highest number of cases was observed at Service 2. Also Service 3 and Surgery Service showed some cases. The most common sites of infection were respiratory system and skin (surgical wounds). Aerobic gramnegative bacteria were the most frequently isolated 78,17% grampositive 17,3% and candida sp. 4,3%. Pseudomonas aeruginosa had a multiresistance pattern.