In Venezuela a chewing-type tobacco is called chimo, which is used by persons of different age and socioeconomic status. Chimo like all tobacco derivatives is addictive and has been linked to oral precancerous lesions. The nicotine content of chimo is poorly documented, given its high consumption it was decided to determine the level of nicotine in six commercial varieties available in the Venezuelan market. Gas chromatography coupled to mass spectrometer (GC-MS) was used in this study. It was found that artisan chimo contained 0.009 mg/g of nicotine while semi-industrial chimo contained 10.8 mg/g (Chinata®), 8.5 mg/g (Recio Apureno®), 4.04 mg/g (Llanero de Socopo®), 3.84 mg/g (Tigrito®) y 3.64 mg/g (Andinito®). These differences in nicotine level might be due either the tobacco varieties used or to different amounts of tobacco leaves used in the manufacture of each variety of chimo.