To conserve biodiversity, the conjunct construction of knowledge and information by the variety of groups of actors involved is fundamental. An analysis of the bibliographic production in Colombia between 1993 and 2013 in the framework of the Estrategia Nacional para la Conservacion de Plantas (National Strategy for Plant Conservation-NSPC-) is presented here. A database of publications was constructed for these 20 years through consultation using keywords related to each one of the 16 targets established by the NSPC. For the analysis, publications were classified according to the target of the NSPC to which they mostly contribute, type of publication, natural geographic area, publishing institution and human groups involved. We recorded 1,805 publications, including books, articles in indexed journals, theses, reports, and booklets. Target 1 of the NSPC related to inventories and floristic lists presented the highest number of records whereas targets 2, 5, 10, and 11, which are related to the conservation status of plants, important areas for plant conservation, invasive species, and international trafficking of species presented the lowest number of publications. 60 % of the publications were concentrated in the Andean region, and the Orinoquia and Caribbean regions had the lowest representation. Based on these results, a set of recommendations are presented to face the most challenging issues related to achieving NSPC targets.