During the massive gametes release of hermatypic corals on 5, 6 and 7 september 2012 in Isla Grande, Archipelago de Nuestra Senora del Rosario and into the framework of the effects of runoff over recruitment of scleractinian corals, was carried out assessing the composition and abundance of the zooplankton community in day and night sessions before, during and after the event, by analyzing samples of surface zooplankton collected with buckets and filtered through zooplankton net. Given the complexity of identifying, the categories family-taxon and taxon-morphotype were established. In total 34 families belonging to 19 orders and 8 phyla were identified, being the subphylum Crustacea with the most families (23), representing 67.67% of the relative abundances of sampling. During the day of the pre spawning were highlighted Miraciidae (13.79%) family and Oikopleuridae (23.91%) at night; in the daytime spawning Oikopleuridae (24.93%) and in the night Palaemonidae (21.81%); and on both days of post spawning Oikopleuridae (29.88% and 15.81%, respectively). Within the taxon-morphotype classification, the deformed normal morphotypes, morula and eggs were found to be more abundant during the spawning and post spawning; while planulae larvae were significant in the post spawning day. The classification analysis established the separation of groups by days. The presence of families Oikopleuridae, Paracalanidae, Corycaeidae, Centropagidae and Cumacea, provided the greatest similarity. According to ecological attributes, the night shift was characterized by the greatest richness of families and total abundance, being more diverse, with high uniformity and low prevalence compared to daytime.