This article is the first report of equine cholangiocarcinoma in Colombia. A 14 years old horse was referred to the Large animal Clinic at the national University of Colombia, with history of weight loss; on examination, the horse was letarghic, in poor body condition, with abdominal distention and ventral edema. The clinical examination suggested a hepatic tumor as a very probable diagnosis; the clinical pathology results showed severe involve - ment of biliary ducts and hepatic cells. The necropsy and the microscopic examen revealed cholangiocarcinoma.