Endophytic fungi of tropical orchids have been relatively understudied and could end up having an interesting series of applications in orchid conservancy and propagation. One of the less studied groups is the genus Stanhopea. In healthy roots of Stanhopea tricornis we were able to isolate 12 fungi belonging to at least 4 genera: one isolation of Curvularia sp., two of Fusarium sp., two of Stemphylum sp., three of Rhizoctonia sp. and three unidentified fungi were obtained. This report corroborates the presence of endophytic fungi in Stanhopea spp. and states the need of further studies within this important genus.