Clostridium perfringens is considered an important indicator of fecal contamination that can signal the presence of pathogenic microorganisms of difficult and costly detection, and the effectiveness of filtration processes in water purification. This work aimed to verify the methodology of the ISO Standard 14189:2013 for the determination and quantification of C. perfringens in drinking water through the membrane filtration method at the facilities of the Microbiology Laboratory in the Research Group on Environmental Management and Modeling (GAIA), University of Antioquia. The data showed the Laboratory is suitable for obtaining precise and accurate results compared to the referenced acceptance criteria of ISO 14189: 2013 and those of GTC 84, 2003. Accordingly, under the same test conditions, the laboratory is suitable, allowing it to deliver reliable and timely results.