For this study, the effect of different substrates at different proportions was evaluated in physiological, growth and development aspects in plants of the species S. rupestre and R. sativus. For this, under greenhouse conditions in Metro-Verde facilities (Tocancipa), it was stablished the experiment under a completely random block design. The physiological variables: Relative water content (RWC), Water potential (?w), Maximum quantum efficiency of PSII (Fv/Fm), Photochemical quenching (qP), Non-photochemical quenching (NPQ), maximum quantum yield of PSII under light condition (YII), growth variables: Leaf dry weight (LDW), Stem dry weight (SDW), Root dry weight (RDW), Total dry weight (TDW), Leaf area (LA), and the growth and development indexes, Leaf area ratio (LAR), Specific leaf area (SLA), Leaf weight relation (LWR) and Root weight relation (RWR), were measured in plants cultivated in volume to volume (v/v) mixes of the materials Carbonite (CA), Compost (C) and Pine Bark (CP) in the respective proportions for each treatment (1:1:2, 3:2:5, 3.5:1.5:5, 4:1:5), and a control mixture composed of Burned rice husk (CQ), Carbonite and Compost in a proportion of 1:1:1 (v/v). For S. rupestre plants the mixture 25CA-25C-50CP influenced the best response for the physiological and growth variables and the growth and development indexes, specially an optimal value of RWC (65%), while for R. sativus plants, the mixtures 30CA-20C-50CP y 40CA-10C-50CP influenced the best response for the physiological and growth variables including the growth and development indexes, especially for the variables RWC (91.2% and 84.5%), Fv/Fm (0.77 and 0.81) and RDW (0.99g y 0.78g).