The genus Fusarium comprises a group of soil-borne species which have been known as vascular wilt causing agents on a wide range of economically important crops. Recently, Fusarium has been reported as an emerging opportunistic pathogen in immunocompromised humans and it has been also associated to superficial mycoses in animals and humans. Given this ability to cause disease on different hosts, Fusarium could be studied as a multihost pathogen model. The present study evaluated the pathogenicity of 12 Fusarium spp. isolates, from plants, animals and humans, on Dianthus caryophyllus (carnation), Lycopersicon esculentum (tomato) y Passiflora edulis Sims (passion fruit). Vascular wilt symptoms were weekly evaluated during two months under greenhouse conditions. After this period of time, vascular necrosis, stem colonization, stem necrotic spots and roots rot were determined. Not all typical symptoms of vascular wilt were determined on the three plant species tested, the plants mainly showed chlorosis and defoliation. All isolates caused vascular necrosis and necrotic spots, as well as they were recovered from stem vascular bundles. Because the Fusarium spp. isolates evaluated in the study were able to establish the infection and showed some symptoms of vascular wilt on carnation, tomato and passion fruit plants, regardless their species or isolation origin, Fusarium spp. could be considered as a possible model for multihost pathogens.