The Epstein Barr virus (EBV) has a high prevalence in humans. It is associated with some cancers related to B lymphocytes. This study aimed to determine its presence in canine lymphomas and selected 20 cases of diagnosed lymphoma. Initially lymphomas were reclassified according to the new histopathological classifications of lymphomas and were immunomarked with anti-CD79a, anti-CD3 and anti-LMP-1 antibodies. Results showed that the most common lymphomas were nodal lymphoma of the T zone in 75%. We found 18 cases positive to CD3, 2 cases positive to CD79a and 6 cases positive to LMP-1 representing 30% of positivity of EBV in lymphomas. Statistical analysis showed high significance between the presence of the virus and the lymphoma. The Golden Retriever breed was related to the presence of lymphoma, but not to the presence of the virus. This is one of the few studies where EBV is sought in canines and a relationship between their presence and the presence of lymphoma is demonstrated.