The present study evaluated the physicochemical and biological quality of the Quebrada La Colorada in different pluviometric periods of the years 2011, 2014 and 2016, in the municipality of Villa de Leyva, Boyaca - Colombia, through monitoring physicochemical characteristics and the biological community of macroinvertebrates. Two sampling stations were selected in the system, one in tropical forest areas restored after forest fires, and the other in unrestricted areas adjacent. The physicochemical parameters evaluated were dissolved oxygen, pH, temperature, nitrites, nitrates, phosphates and conductivity, and the analysis of the community of macroinvertebrates was performed through biotic indexes of diversity, dominance and similarity. For the two study areas four classes were recorded (Gastropoda, Arachnida, Malacostraca, Clitellata and Insecta); 8 orders, 26 families and 25 macroinvertebrate morphotypes. As a result of the physical-chemical and biological quality analysis in the La Colorada quebrada for the study periods, an improvement was obtained in the water quality, where it is concluded that the restoration process has influence on the water system, Since this study showed a positive impact on some actions such as biological diversity, change in the physicochemical structure of the system, variation of microhabitats, changes in rainfall history of sampling times, nutrient contribution and productive capacity of the ecosystem. Keywords: Ecological restoration, water quality, physicochemical parameters and macroinvertebrates.