Introduction: A very common practice in agriculture is the disposal of wastewater and biosolid from water treatment systems. Due to their high nutrient content crop yields improve substantially. However, occurrence of fecal origin pathogens produces sanitary risk to farmers and consumers. Objective: To determine occurrence and concentration of helminth eggs in irrigation waters, biosolids, agricultural soils and grasses. Materials and methods: Water (NMX 113-SCFI, 1999), biosolid, soil (NOM-004 ECOL, 2002) and grass samples (Kozan et al., 2005), were collected for HE analysis. Behavior of HE was evaluated in irrigation waters and dairy cattle grassland were the organic amendment applied was the biosolid. Results. In water were found concentrations between 0,1-3 Total helminth eggs/L (THE) and 0,1-1 Viable helminth eggs/L (VHE), in biosolid and soil 3-22 THE/4 g Dry weight (DW) and 2-12 VHE/g DW and in grass <2-9 THE/ g Fresh weight (FW) and <1-3 VHE/ g FW. HE permanence in each matrix varies from days to months, representing a sanitary risk to the farmers as well as to consumers. Conclusions . The presence of helminth eggs at the assessed matrices confirms sanitary risk as a consequence of such practices. Therefore, is vital to control and incorporate regulations related to wastewater and biosolids usage in agriculture.