Objective: To detect Staphylococcus aureus and Streptococcus pyogenes in health personnel of the surgical and surgery services at Hospital San Jose. Methodology. Descriptive, Prospective cross-sectional study. The techniques used were surveys and sampling nasal and pharyngeal microbiological cultures. Results. It was found that from 29 persons under study, 10 (34.40yo) were S. aureus carriers, and it was not found S. pyogenes carriers. From the positives, 8 (80%) were S. aureus nasal carriers, and 2 (20%) pharyngeal carriers. From 8 people (80%), 4 (40%) belonged to the department ofsurgery and another 4 (40%) to the surgical services; 2 (20%) from the pharyngeal positives worked at the surgery services. From the carriers, 5 people (50%) were nursing assistants, followed by 4 (40%), who belong to doctors and 1 person (10%) belonged to nursing.