The eucalyptus tree (Eucalyptus pellita) is a forester source that represents an important opportunity for the reforestation development in the Colombian Orinoquia. For the clonal propagation of trees selected by their excellent characteristics, growth and quality of the wood is required to develop efficient multiplication strategies. The objective of the present work was to propagate plustrees of E. pellita F. Muell, by techniques of plant tissue culture in vitro. For the establishment phase, nodal segments obtained from epicormic buds of plus trees were used as source of explants. Under aseptic conditions explants were disinfected with 70% ethanol during one minute, followed by immersion in 1% sodium hypochlorite during 15 minutes. Explants were planted in Murashige and Skoog media, obtaining up to 70% of the cultures free of contamination. For