The uses of ultrasounds in medical applications like ultrasonic surgery, diagnosis, and nowadays in cellular separations and manipulations, has foment the study about physical and biological effects, related to exposure of cells at an acoustic field. The interest in determining these effects could help to optimize the ultrasound uses, and identify new applications. In order to improve the uses in applications where in vitro cell cultures are used, this manuscript review the effects generated in cells when are exposed to ultrasonic fields.