The objetives of this work were the collection and stablishment of a garden of albahaca and a contribution to the botanical characteristic of the cultivars introduced in the germoplasm bank .The collection was gathered at cultivated fields, natural vegetation areas, house gardens and local markets. Seventeen (17) species were collected, all belonging to the generous Ocimum from six different zones of Valle del Cauca. Three species are cultivated (O. basilium L., O. americanum Jack Host y O. minimum L.) and two are considered ornamentals (O. gratissimum Hook y O. micranthum Willd). Phenotipicaly, high variety was observed in the plant architecture, in its pubescence presence, in the type of inflorescence and in flowers, leaves and fruits.