Choco is one of the departments with the highest malaria burden brings in Colombia. Urban and rural areas of Quibdo municipality have the highest number of permanent inhabitants in the department and also a permanent floating population coming from malaria hyperendemic areas. Because urban malaria transmission has been reported on Quibdo this study was carried out for seven 12 Determinacion del papel en la transmision de malaria de las especies de Anopheles months to provide insight into some biology aspects of local Anopheles spp. in Pacurita, a small town located 7km from Quibdo, where gold mining activities are generating enabling environments for new larval habitats. Anopheles breeding sites were indentified and characterized; and standard dipping method was used to collect immature forms; only 2 species were represented on the inspected breeding sites, An. nuneztovari Gabaldon 1940 (n=16; 3 Gold mining excavations –GME-) and An. darlingi Root 1926 (n=1; 1 GME). Human landing catch method (138 hours in indoor, 161 hours in outdoor) resulted on 7 species landing on human; An. apicimacula Dyar & Knab 1906 (n=1), An. darlingi (n=22), An. neivai Dyar & Knab 1913 (n=6), An. nuneztovari [n=188; indoor biting activity peaks 2122H, 00-01H, 03-04H and 05-06H; outdoor biting activity showed activity peaks from 19-20H, 22-23H and 00-01H], An. oswaldoi sl Peryassu, 1922 (n=5), An. rangeli Gabaldon, Cova Garcia & Lopez, 1940 (n=9) and An. triannulatus sl Neiva & Pinto 1922 (n=13). Despite the fact that malaria cases occurred during this study, CS-protein ELISA assay did not detect any individuals infected with Plasmodium spp. Because of the observed biting activity of An. nuneztovari, considered a primary vector in Choco, vector control could include long lasting insecticide-treated nets; use of repellants during outdoor and daytime activities, and strengthening accurate malaria diagnosis and early treatment at the local level.