A cryopreservation method was evaluated on a sample of Colombian ecotype Physalis peruviana using the encapsulation-dehydration technique. Sucrose preculture conditions of the apex were evaluated, obtaining the best results 54% regrowth with a two step preculture of 0.087M and 0.174M two day each. These were also grown under different light and temperature conditions obtaining best results in cold 4?C and darkness. For dehydration and osmoprotection two concentrations of sucrose 0.75M and 1.0M supplemented liquid medium for 24 hours was evaluated. In the absence of preculture conditions the high concentrations of sucrose resulted toxic on the tissues, inhibiting their regrowth and survival. Preculture with sucrose allowed the conditioning to the osmoprotection treatment, which was achieved with a concentration of 0.75M sucrose, minimizing final moist content of the beads to ?15%. The beads frozen in liquid nitrogen and thawed, increased their survival and regrowth up to 30% more when rehydrated, but reactivation y regeneration was only possible with regeneration medium supplemented with BAP under low light conditions. A survival of 100% of the cryopreserved beads was obtained, and direct growth withouth callus formation was observed in every case. Results obtained in the present study proof the success of encapsulation-dehydration technique for a herbaceous tropical species, y serves as model for the establishment of future protocols.