Malassezia spp. yeast is characterized by being lipodependiente, necessitating the incorporation of lipidic substances in the culture media, making the susceptibility testing in vitro, since its physiology is impossible using standard protocols as M27-A proposed by CLSI (Clinical and Laboratory Standards Institute). Given this physiological characteristic of interest and testing the in vitro susceptibility to clinical isolates obtained from humans and animals, it is proposed to evaluate the inoculum and the culture medium for the growth of the microorganism. For the inoculum, was determined emulsifier which to obtain the lowest low cell clumping treatments Tween 80 0.1, 0.5 and 1% Tween 40 at 0.5 and 1%, 1% Glycerol 0.05% Triton. To the culture medium was evaluated three base medium: Christensen's urea broth base, Christensen urea broth base supplemented with sterile solution of urea and Sabouraud broth with different lipid supplements cut by a factor (Tween 40, Tween 60, Tween 80 and glycerol). The quantification of biomass was performed using spectrophotometric reading at a wavelength of 530 nm. The emulsifier of inoculum to obtain homogenous cell suspensions was distilled with 1% Tween 80. As the culture medium was set Sabouraud broth supplemented with 0.5% Tween 40 and 0.5% Tween 60 with an incubation time of 96 hours.