Indiscriminate use of chemical fertilizers has contributed to deterioration of biological, physical and chemical properties of soil, resulting in loss of productive capacity of soils. For this reason the use of biofertilizers, has emerged as an alternative technology. The objective of this research was to develop a suitable multiplication system in liquid fermentation for Azotobacter chroococcum AC1 strain. Sequential statistical designs where use for determinate conditions in fermentation system. Interaction between agitation, aeration and pH was evaluated on viable biomass (CFU / mL) of AC1. Besides the encystment ability of AC1 strain was evaluated using two encystment agents. PGPR activities in bacteria was evaluated by assaying nitrogen fixation by ARA technique, phosphorus solubilization by phosphomolybdenum blue technique and indolic compound production by colorimetric technique reaction using Salkowsky’s reagent. Results showed dual and tripartite interactions between pH, aeration and agitation, showed significant (p <0.05) in the three conditions tested individually in a dual interaction and tripartite interaction, with positive aeration and agitation. The addition of encystment agents evaluated, AE01 and AE02, demonstrated the ability to form cysts for AC1 strain under stress conditions. Finally, fermentation and encystment conditions not affect biological activities tested