Volatile secondary metabolites obtained from the leaves and flowers (fresh and dried) of Chromolaena barranquillensis (native specie of the Departamento del Atlantico), and isolated by simultaneous-distillation extraction (SDE) and conventional hydrodistillation (HD), were characterized chemically by gas chromatography coupled with mass spectrometry (GC-MS). The main compounds identified in the volatile fractions were: (a). fresh flowers – -elemene (24.4%), -pinene (19.6%), and limonene (15.7%); (b). dried flowers – -caryophyllene (21.4%), germacrene D (16.6%), and caryophyllene oxide (13.6%); (c). fresh leaves – myrcene (39.0%), -curcumene (17.8%), and limonene (10.2%); and, (d). dried leaves – -caryophyllene (13.8%), -curcumene (9.8%), elemene (7.7%), and caryophyllene oxide (6.4%). In the essential oils (EO) were recognized as major components: (e). fresh flowers – -caryophyllene (22.9%), -elemene (14.3%), and germacrene D (14.0%); (f). dried flowers – -caryophyllene (23.6%), -elemene (20.6%), and germacrene D (15.8%); (g). fresh leaves – -caryophyllene (22.0%), limonene (11.8%), -cadinene (6.8%), and germacrene D (6.1%); and, (h). dried leaves – -caryophyllene (29.1%), germacrene D (13.1%), and caryophyllene oxide (12.0%). The yields achieved in the isolation of EO were 0.2%/0.4% and 0.06%/0.1% for fresh/dried flowers and fresh/dried leaves, respectively.