Objective: To describe the microbiological profile of ventilator-associated pneumonia (VAP) at the intensive care unit (ICU) of the Clinica Universitaria Bolivariana from 2009 to 2011. Methodology: A single-center retrospective study was conducted. Endotracheal aspirate was used for microbiological diagnosis. Quantitative variables were described using measures of central tendency, (mean or median) and dispersion, such as standard deviation or range. Categorical variables were analyzed as proportions. Results: Thirty-four patients were included, of which 19 were men (56%); the mean age was 60 (SD17.75); the median APACHE II score was 17 (8-36). The most common diagnoses upon admission were lung disease (24%), sepsis (18%), and trauma (18%). Klebsiella pneumoniae (23%) and Pseudomonas aeruginosa (21%) were the most common germs. We found a correlation of 59% between the isolates and germs associated with late VAP. Streptococcus viridans was the principal germ (20%) in throat cultures, followed by K. pneumoniae (16%) and E. Coli (16%). There was only a 38% correlation between the throat culture and endotracheal aspirates. Conclusions: K. pneumoniae and P. aeruginosa germs were the most frequent, with a poor correlation between the throat cultures and endotracheal aspirates.