The crude extracts of three species of plants belonging to the family Melastomataceae and a species belonging to the family Rubiaceae were obtained in aqueous phase and butanol, using solid-liquid extractions and liquid-liquid. The in vitro antifungal activity of these extracts to concentrations of 100000, 50000, 25000, 10000, 5000 and 2500 mg/L in 99% DMSO was evaluated against the fungus Aspergillus niger and yeast Candida albicans using the technique of agar diffusion. Given the non-distribution of the extracts in the culture medium led to the determination by the method microdilution with MTT, which react to long-term commitment to the culture medium used (Sabouraud 0.5%) giving coloration even in the absence of inoculum. Finally the antifungal activity of these extracts was evaluated by macrodilucion in tubes at concentrations of 5%, 3% and 1% m/v against Candida albicans to an absorbance of 0.1 (λ = 495 nm) and Aspergillus niger to 0.05% m /v. Concluding that the aqueous extract of Miconia sp 5%, Miconia caudata 5%, Clidemia hirta (3 and 5) % and extracts butanol from Miconia sp (3 and 5) %, Miconia caudata (3 and 5) %, Clidemia hirta 5% And Hamelia patens (1, 3 and 5) % antifungal activity showed opposite Candida albicans. Contrary to this none of the extracts evaluated presenting antifungal activity in front of Aspergillus niger.