Piper Pesaresanum and Piper crassinervium are two vegetable spices belonging to the genus Piper and the family Piperaceae, that have been studied by their wide global distribution and their biologic activity. In this study, it was evaluated the antibacterial activity of the hexane, dichloromethane, methanol, and water - methanol (9:1) extracts of the mentioned spices, by the well diffusion method, finding that the dichloromethane extract of P. Pesaresanum and its polar fractions obtained by column chromatography in silica gel, were active against Staphylococcus aureus. In addition, it was evaluated the antifungal activity of the these extracts, against Mycosphaerella fijiensis Morelet fungus in its sexual and asexual phases by the germinative tube elongation and radial growth methods respectively, where it was found that the methanolic extract of the P. Pesaresanum inhibited the ascospores germination 100 % in the sexual phase, while the hexane extract of the same plant showed 100% of the activity against the asexual phase of the M. fijiensis. A chromatography profile by HPLC–MS was made to the dichloromethane and methanol extracts of the P. pesaresanum that were active, in which highlights the presence of various polar compounds. Also was identified a similar structural features piperamida the pipercida. This info was complemented by analysis of nuclear magnetic resonance spectra 1H- NMR and COSY 1H-1H.