The plant species studied in this work were selected through surveys of medicinal plant made to healers traders in the cities of Pereira and Santa Rosa de Cabal (Risaralda) to select the most widely prescribed; as a result of this survey the species cidron (Aloysia triphylla), malva (Malva sylvestris), toronjil (Melissa officinalis), perejil (Petroselinum sativum) and ortiga (Urtica dioica) were the most commonly marketed. From these plant were obtained the n-hexane, dichloromethane and methanol extracts, on which was evaluated the antibacterial activity against Bacillus subtilis, Candida albicans, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus by using the method of agar plate diffusion. However, none of the plant extracts showed significant activity at the concentrations evaluated (250-4000 mg/L). Also, the antioxidant capacity was determined using the technique of radical discoloration with the 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical. Of the 15 extracts tested, 66.7% presented antioxidant activity higher to 25% and in these extracts were found high contents of triterpenes, phenols, tannins and saponins. Melissa officinalis showed the highest percentage of antioxidant activity with values of 50.3, 48.9 and 49.2% for the n-hexane, dichloromethane and methanol extracts, respectively. In addition, a phytochemical screening was performed using thin layer choromatography (TLC) to determine the presence of: alkaloids, sterols, terpens, saponins, phenols, tannins, flavonoids and amides in the extracts obtained.