The allelic frequencies of 14 polymorphic loci were obtained for the Paisa community (Antioquia, Colombia) and compared with those of Caucasoids, Negroids and Amerindians as an approach to better understand its ancestral origin. Data pertaining to Caucasoids included Basques, Spaniards and Jews, historically assumed to be the ancestors of Paisas. These comparisons suggested that the Paisa community is a Caucasoid group with very low Amerindian or Negroid contributions. Spaniards or Basques and, to a lesser extent, Sephardims seem to have been their most probable ancestors.