In order to evaluate the effect of earthworm manure on the growth of coffee seedlings in eight soils from the Colombian coffee region with different organic matter (OM) content, an experiment was carried out at Cenicafe (Chinchina, Caldas). Four proportions of earthworm manure mixed with soil were evaluated: 0, 25, 50, and 75%. For each proportion, fifteen nursery bags were filled and one Caturra variety coffee seedling was planted per bag. After six months, root and shoot dry weight were measured. In seven soils, the proportion 25% of earthworm manure increased the dry weight of plants between 180 to 1500% compared to that of soil without manure and regardless the soil OM contents. The differences in its response magnitude were associated with changes in pH as well as in Cu and K contents of the substrate. Proportions 50 and 75% of earthworm manure affected negatively the plants growth in all the soils.