Adventitious regeneration is a key step in the application of genetic engineering to the breeding programs of plants. In this work a method for adventitious shoot regeneration from leaves of micropropagated guava shoots has been developed and some of main factors to affect the shoot regeneration like, concentration of plant growth regulators (citoquinine), physiological state of explants and the wound are studied. Leaves from guavainvitro cultured of variety Cuban Red Dwarf 18-40 was used like explant in all experiments. The best result was reached with MS basal medium supplemented with 0.75 mg/L of 6-benzylaminopurine. Was demonstrated that for the leaves with more multiplication subculture number and taken from lower part of shoot (older); the morfogenetic potential falls significantly (p 60%) and the largest number of shoot per regenerating leaf (>3) were obtained with several wounds ca