Compared to optimal Zn supply, certain concentration of low Zinc was found to injure maize (Zea mays L.) growth more severely than Zn deficiency treatment in solution culture experiment on Shendan10 (SD10). A further analysis on POD (peroxidase), SOD (superoxide dismutase), CAT (catalase) isozymes, and protein expression in SDSPAGE was discussed in this discourse and conclusions are drawn as follows: plants grown in the low Zinc nutrition solution (about 0.001 μmol/L) were stunted severest, and more visible deficiency symptoms appeared in shoot than that in root. The above three isozyme and protein expressions presented obvious difference under low Zn and Zn deficiency treatment from that at optimal Zn supply. At certain low Zn, some isozyme expressions were enhanced or specifically induced while some were inhibited. Under low Zn and Zn deficiency treatment, some new protein components occurred, while some original components were lost. However, low Zn caused more protein components disappeared. These changes may have a close relationship with maize growth and injury.